1366 Age-related changes of type VI collagen in the dermis and its relationship with G0-phase of the fibroblast cell cycle

The cell cycle is divided into the active phase (G1/S/G2/M) and G0-phase (G0) in which division stops with respect to division. Low gene expression level common G0 due stops, whereas G0-unique characteristics are known liver nerves. Therefore, we investigated G0-specific Normal human dermal fibroblasts (HFs) has been reported many of them G1/G0 when contacting collagen scaffold. In this study, used proliferation marker Ki67 identify Ki67-negative cells as cells. G0-cells rich HFs culture was induced by serum starvation. Changes mRNA or protein levels were evaluated DNA microarray real time RT-PCR, immunohisto/cytochemistry, respectively. results indicated that (MKI67) decreased under starvation, confirming induction culture. Most genes related extracellular matrix (ECM) components decreased, however, several upregulated including type VI (COL6). And then, focused on further up-regulated COL6 culture, because recently shown be involved a wide range tissues pathologies. up-regulations confirmed both levels. Furthermore, examined age-related changes using skin explant. results, significantly aging, interestingly, phenomenon remarkable reticular dermis, more than papillary dermis. These suggested production decreases aging. Altogether, focusing cells, have discovered decrease also affects physiological functions such autophagy, expected new anti-aging target.